![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4724091:Design
Designed by: Qingqing Zheng Group: iGEM23_NJTech-China-A (2023-10-12)
IsPETaseS93_I94insE/W159H-Linker
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 549 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We connected Linker behind the double mutant to facilitate domain fusion.
Source
It's a new part, which is a double mutant of IsPETase from Ideonella sakaiensis.